Center on the Biology of Aging

Graduated: April 2018
Current location: Sanofi Genzyme, Framingham, MA

Mentor: Marc Tatar

Description of trainee's research project and progress: Stephanie Post was a student in the lab of Professor Marc Tatar. Her project investigated the redundant and unique functions of Drosophila insulin-like peptides (Dilps), and the mechanisms by which two hormones can regulate different organismal phenotypes through a common receptor. Stephanie matriculated in the MCB Program in September 2012. She was supported for two years by this T32 (09/01/13-08/31/15). She successfully defended her Ph.D. on April 13, 2018 (time to Ph.D. defense, 5.7 years). She contributed to the publication of nine papers while at Brown. She subsequently pursued postdoctoral studies at Sanofi Genzyme. She currently holds the position of Staff Scientist at Sanofi Genzyme.

Publications:
1) Tatar M, Post S, Yu K (2014). Nutrient control of Drosophila longevity. Trends Endocrinol. Metab. 25: 509-17.

2) Bai H, Post S, Kang P, Tatar M (2015). Drosophila longevity assurance conferred by reduced insulin receptor substrate chico partially requires d4eBP. PLoS One 10: e0134415.

3) Post S, Tatar M (2016). Nutritional geometric profiles of insulin/IGF expression in Drosophila melanogaster. PLoSOne 11: e0155628.

4) Adebayo Michael AO, Ahsan N, Zabala V, Francois-Vaughan H, Post S, Brilliant KE, Salomon AR, Sanders JA, Gruppuso PA. Proteomic Analysis of Laser Capture Microdissected Focal Lesions in a Rat Model of Progenitor Marker-Positive Hepatocellular Carcinoma (2017). Oncotarget 8(16):26041-56. PMC5432236.

5) Chavez JD, Eng JK, Schweppe DK, Cilia M, Rivera K, Zhong X, Wu X, Allen T, Khurgel M, Kumar A, LampropoulosA, Larsson M, Maity S, Morozov Y, Pathmasiri W, Perez-Neut M, Pineyro-Ruiz C, Polina E; Post S, Rider M,; Tokmina-Roszyk D; Viera Parrine Sant'Ana D; Tyson K, Bruce JE. A general method for targeted quantitative cross-linking mass spectrometry. PLoS One 11(12):e0167547. PMC5172568.

6) Lin F, Hossain MA, Post S, Karashchuk G, Tatar M, De Meytz P, Wade JM (2016). Total Solid-Phase Synthesis of Biologically Active Drosophila Insulin-Like Peptide 2 (DILP2). Aust J Chem. 70(2):208-212.

7) Kang P, Chang K, Liu Y, Bouska M, Birnbaum A, Karashchuk G, Thakore R, Zheng W, Post S, Brent CS, Li S, Tatar M, Bai H (2017). Drosophila Kruppel homolog 1 represses lipolysis through interaction with dFOXO. Sci Rep. 7: 16369. PMC5703730.

8) Post S, Karashchuk G, Wade JM, De Meyts P, Sajid W, Tatar M. Drosophila insulin-like peptides DILP2 and DILP5 induce unique signaling events and GlyP phosphorylation that is required for dilp2 to regulate longevity. In preparation.

9) Post S, Liao S, Yamamoto R, Nassel D, Tatar M. Drosophila melanogaster insulin-like peptide dilp1 is sufficient and required for dilp2 to regulate lifespan and metabolism. In preparation.

 Conference presentations:

1) Post S, Tatar M. The nutritional geometry of insulin shapes the landscape of aging. Annual Drosophila Research Conference, San Diego, CA, March 26-30, 2014, (Abstract-poster).

2) Post S, Wade J, De Meyts P, Sajid W, Tatar M. Identifying unique functions of D. melanogaster insulin-like peptides. Gordon Research Conference on IGF and Insulin System in Physiology and Disease, Ventura Beach, CA, March 8-13, 2015, (Abstract-poster).

3) Post S, Wade J, De Meyts P, Sajid W, Tatar M. A tale of two peptides: Characterizing cellular responses of D. melanogaster insulin-like peptides. FASEB Research Conference on Protein Kinase Signaling Network Regulation, Snowmass, CO, July 17-22, 2016, (Short talk selected from abstracts).

 4) Post S, Wade J, Sajid W, De Meyts P, Tatar, M. Profiling insulin/IGF stimulated phosphorylation and transcription to characterize the functions of Drosophila insulin-like peptides. Next Generation Dx Summit, Washington DC, August 15-18, 2017, (Abstract-poster).

 5) Post S, Wade J, Sajid W, De Meyts P, Tatar, M. Drosophila insulin-like peptides DILP2 and DILP5 regulate distinct cell signaling events that differentially contribute to metabolism and aging. Northeast Glenn Meeting on the Biology of Aging, UConn, Farmington, CT, Nov 9, 2017, (Short talk selected from abstracts).

Honors: 

1.    Best poster award, MCB Graduate Program retreat, 2015
2.   AFAR/Glenn Scholarship for Research in the Biology of Aging, 2017